In the phosphorescence lifetime measurements with pulse method, both the excitation light pulse parameters and initial time selected for intensity decay analysis are crucial to the accurate lifetime calculation. 脉冲法磷光寿命测量中,激发光脉冲参量(脉冲宽度和形状)、用于磷光强度衰减分析的起始时刻的选取是影响寿命计算准确度的关键因素。
The relation between phosphorescence intensity decay and the parameters of the excitation light pulse is analyzed based on the linear time invariance. it is found that the phosphorescence intensity decay is independent on the parameters of the excitation light pulse after the excitation light vanishing. 以线性时不变系统理论为依据,探讨了磷光强度衰减特性与激发光脉冲参量之间的关系,发现对于有限宽度的激发光,激发光脉冲消失之后的磷光强度衰减特性独立于激发光脉冲参量;
The light intensity of the excitation light and the phosphorescence is simultaneously recorded in terms of gray value by the confocal microscopy system. The phosphorescence lifetime measurement can be realized with time resolved analysis for the phosphorescence intensity decay. 共焦成像系统以灰度值同时记录了激发光和样品发射磷光的强度变化信息,通过对磷光强度衰减的时间分辨分析,实现对磷光寿命的测量。